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+In additional complete studies, it was confirmed that, in vitro, the tandem Tudor area of 53BP1 binds to a number of dimethylated lysine residues on the p53 C-terminus, with a choice for p53K382me2 172 , 173 The KD worth of 53BP1 Tudor-p53K382me2 is about zero.9 µM, whereas that of 53BP1 Tudor-p53K372me2 is 20 µM, indicating that the binding of 53BP1 Tudor to p53K382me2 is way stronger 173 Further, technology of K382me2 in the p53K370R mutant retains the power of p53 protein to work together with 53BP1 in vivo, suggesting that p53 K382 dimethylation is more doubtless the primary target of 53BP1 Tudor domains 172 Since different regions of p53 and 53BP1 also work together, further study is required to outline the specificity and capabilities of p53 C-terminal lysine methylation for 53BP1 affiliation and other actions.
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